The rules require clubs to include in their starting line-ups at least six of the 11 players that have started most often in first team games this season.
First-choice goalkeeper Rob Elliot is out for six weeks with an adductor muscle injury but on-loan Wolves goalkeeper Carl Ikeme is able to play.
Ikeme and fellow loanee David Mooney were made ineligible for the FA Cup defeat by their parent clubs but will be back for the southern quarter-final tie at St Mary's.
Southampton manager Alan Pardew will welcome back Jacob Mellis and Neal Trotman.
The on-loan pair are available again for the visit of the Addicks, having been ruled out by parent clubs Chelsea and Preston for Saints' 3-2 FA Cup first round win against Bristol Rovers.
But Pardew will be without Oscar Gobern, who played in the cup tie on Friday evening. The 18-year-old is cup-tied having featured for MK Dons in the Johnstone's Paint Trophy during a loan spell earlier in the season.
Graeme Murty is also ruled out of the match with a long-standing knee ligament injury.
However, Pardew will be boosted by the news that the former Reading captain will be back in training in the next couple of days.
Sporting Life